CML (Carboxymethyl Lysine) ELISA Kit

• Description :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CML protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CML. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CML in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  N (6) -Carboxymethyllysine

• Reactivity :

  General

• Field of Research :

  Metabolic pathway; Endocrinology; Cardiovascular biology; Genetic science; Nutrition metabolism

• Assay Type :

  Competitive Inhibition

• Assay Principle :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CML. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CML. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CML in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  2h

• Standard :

  5000 ng/mL

• Sample Type :

  Serum, plasma and other biological fluids

• Detection Range :

  78.13-5000 ng/mL

• Sensitivity :

  24.7 ng/mL