Dog TRAIL (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) ELISA Kit

• Description :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog TRAIL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog TRAIL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog TRAIL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog TRAIL in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  CD253; TNFSF10; APO2L; Apo2-L; TL2; TRAIL; Tumor Necrosis Factor Ligand Superfamily Member 10; Apo-2 Ligand

• Reactivity :

  Dog

• Field of Research :

  Apoptosis; Tumor immunity

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog TRAIL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog TRAIL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog TRAIL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog TRAIL in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  10 ng/mL

• Sample Type :

  Tissue homogenates, cell lysates and other biological fluids.

• Detection Range :

  0.16-10 ng/mL

• Sensitivity :

  0.057 ng/mL