Cattle TIMP4 (Tissue Inhibitors Of Metalloproteinase 4) ELISA Kit

• Description :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle TIMP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TIMP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle TIMP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TIMP4 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  TIMP Metallopeptidase Inhibitor 4; Tissue Inhibitor of Metalloproteinases 4

• UniProt :

  O97563

• Reactivity :

  Cattle

• Field of Research :

  Tumor immunity; Cardiovascular biology

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle TIMP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TIMP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle TIMP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TIMP4 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  20 ng/mL

• Sample Type :

  Serum, plasma and other biological fluids.

• Detection Range :

  0.32-20 ng/mL

• Sensitivity :

  0.127 ng/mL