Rat CACT (Carnitine Acylcarnitine Translocase) ELISA Kit

• Description :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CACT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CACT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CACT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CACT in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  SLC25A20; CAC; Solute Carrier Family 25 Member 20; Carnitine Acylcarnitine Carrier; Mitochondrial carnitine/acylcarnitine carrier protein

• UniProt :

  P97521

• Reactivity :

  Rat

• Field of Research :

  Enzyme & Kinase; Hematology; Cardiovascular biology; Bone metabolism

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CACT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CACT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CACT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CACT in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  20 ng/mL

• Sample Type :

  Tissue homogenates and other biological fluids.

• Detection Range :

  0.32-20 ng/mL

• Sensitivity :

  0.132 ng/mL