Human 20S-PSM (20S-Proteasome) ELISA Kit

• Description :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 20S-PSM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 20S-PSM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 20S-PSM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 20S-PSM in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  CP; 20SPSM; 20S Core Particle

• Reactivity :

  Human

• Field of Research :

  Enzyme & Kinase

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 20S-PSM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 20S-PSM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 20S-PSM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 20S-PSM in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  100 ng/mL

• Sample Type :

  Tissue homogenates, cell lysates and other biological fluids

• Detection Range :

  1.57-100 ng/mL

• Sensitivity :

  0.66 ng/mL