Human PRAME (Preferentially Expressed Antigen In Melanoma) ELISA Kit

• Description :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRAME. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PRAME. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PRAME, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRAME in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  MAPE; OIP4; CT130; Cancer/Testis Antigen 130; Opa-interacting protein 4; Melanoma antigen preferentially expressed in tumors

• UniProt :

  P78395

• Reactivity :

  Human

• Field of Research :

  Tumor immunity

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRAME. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PRAME. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PRAME, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRAME in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  10 ng/mL

• Sample Type :

  Tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range :

  0.16-10 ng/mL

• Sensitivity :

  0.063 ng/mL