Human CLEC2C (C-Type Lectin Domain Family 2, Member C) ELISA Kit

• Description :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CLEC2C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CLEC2C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CLEC2C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CLEC2C in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  CD69; CLEC2-C; AIM; p60; EA1; MLR-3; Early T-Cell Activation Antigen p60; Early activation antigen CD69; Activation inducer molecule; Leukocyte surface antigen Leu-23

• UniProt :

  Q07108

• Reactivity :

  Human

• Field of Research :

  Signal transduction; CD & Adhesion molecule; Immune molecule

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CLEC2C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CLEC2C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CLEC2C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CLEC2C in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  1000 pg/mL

• Sample Type :

  Serum, plasma, tissue homogenates, cell lysates and other biological fluids

• Detection Range :

  15.63-1000 pg/mL

• Sensitivity :

  6 pg/mL