Rabbit PAI1 (Plasminogen Activator Inhibitor 1) ELISA Kit

• Description :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit PAI1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit PAI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit PAI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit PAI1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  SERPINE1; PLANH1; Serpin Peptidase Inhibitor Clade E Member 1; Nexin, Plasminogen Activator Inhibitor Type 1 Serpin E1; Endothelial plasminogen activator inhibitor

• Reactivity :

  Rabbit

• Field of Research :

  Metabolic pathway; Hematology

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit PAI1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit PAI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit PAI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit PAI1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  50 ng/mL

• Sample Type :

  Plasma

• Detection Range :

  0.79-50 ng/mL

• Sensitivity :

  0.29 ng/mL