Human KLK8 (Kallikrein 8) ELISA Kit

• Description :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK8 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK8 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  NP; HNP; NRPN; PRSS19; TADG14; BSP1; Neuropsin; Ovasin; Brain Serine Protease 1; Tumor Associated Differentially Expressed Gene 14; Serine protease 19

• UniProt :

  O60259

• Reactivity :

  Human

• Field of Research :

  Enzyme & Kinase; Tumor immunity; Neuro science

• Assay Type :

  Competitive Inhibition

• Assay Principle :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK8 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  2h

• Standard :

  50000 pg/mL

• Sample Type :

  Serum, plasma and other biological fluids

• Detection Range :

  781.25-50000 pg/mL

• Sensitivity :

  216.4 pg/mL