Human Rock2 (Rho Associated Coiled Coil Containing Protein Kinase 2) ELISA Kit

• Description :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Rock-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Rock-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Rock-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Rock-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  ROCK-II

• UniProt :

  O75116

• Reactivity :

  Human

• Field of Research :

  Enzyme & Kinase

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Rock-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Rock-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Rock-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Rock-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  20 ng/ml

• Sample Type :

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range :

  0.32-20 ng/mL

• Sensitivity :

  0.13 ng/mL