Cattle PSPB (Pregnancy Specific Protein B) ELISA Kit

• Description :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle PSPB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle PSPB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle PSPB in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  Pregnancy-associated glycoprotein 1; PAG 1; EC 3.4.23.; Pregnancy-specific protein B; PSP-B

• Reactivity :

  Cattle

• Field of Research :

  Signal transduction; Cardiovascular; Cancer; Metabolism

• Assay Type :

  Competitive Inhibition

• Assay Principle :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle PSPB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle PSPB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle PSPB in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  2.5h

• Standard :

  50 ng/mL

• Sample Type :

  Serum, plasma and other biological fluids

• Detection Range :

  0.78-50 ng/mL

• Sensitivity :

  0.29 ng/mL