EasyStep Human sTFR (Soluble Transferrin Receptor) ELISA Kit

• Product Name Alternative :

  CD71; TFR1; P90; TFRC; T9; Trfr; sTfR; Transferrin receptor protein 1

• Reactivity :

  Human

• Field of Research :

  Signal transduction; CD & Adhesion molecule; Tumor immunity; Infection immunity

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sTFR, and the Human sTFR standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Human sTFR. After TMB substrate solution is added, only those wells that contain Human sTFR and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sTFR in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  1.5h

• Standard :

  5000 ng/mL

• Sample Type :

  Serum, plasma

• Detection Range :

  78.13-5000 ng/mL

• Sensitivity :

  17.55 ng/mL