EasyStep Human CALP (Calprotectin) ELISA Kit

• Product Name Alternative :

  MRP-8/MRP-14; S100A8/A9; CALPRO; Calprotectin

• Reactivity :

  Human

• Field of Research :

  Immunology

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CALP, and the Human CALP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Human CALP. After TMB substrate solution is added, only those wells that contain Human CALP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CALP in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  1.5h

• Standard :

  100 ng/mL

• Sample Type :

  Serum, plasma

• Detection Range :

  1.56-100 ng/mL

• Sensitivity :

  0.57 ng/mL