EasyStep Human T4 (Thyroxine) ELISA Kit

• Description :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human T4 antigen, and the Human T4 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human T4 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human T4 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine

• Reactivity :

  Human

• Field of Research :

  Endocrinology; Hormone metabolism

• Assay Type :

  Competitive Inhibition

• Assay Principle :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human T4 antigen, and the Human T4 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Human T4 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human T4 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  1.5h

• Standard :

  600 ng/mL

• Sample Type :

  Serum, plasma

• Detection Range :

  9.38-600 ng/mL

• Sensitivity :

  7 ng/mL