Human P-Cadherin (Cadherin, Placental) Microsample ELISA Kit

• Product Name Alternative :

  CDH3; CDHP; HJMD; PCAD; Cadherin 3; type 1

• UniProt :

  P22223

• Reactivity :

  Human

• Field of Research :

  Signal Transduction; Neuroscience

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human P-Cadherin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human P-Cadherin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human P-Cadherin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human P-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  4000 pg/mL

• Sample Type :

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range :

  62.50-4000 pg/mL

• Sensitivity :

  36 pg/mL