MAD2 rabbit pAb

• Background :

  MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008]

• Description :

  MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008],

• UniProt :

  Q13257

• Swiss Prot :

  Q13257

• Reactivity :

  Human; Rat; Mouse

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human MAD2L1. AA range:91-140

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; IP; ELISA

• Concentration :

  1 mg/ml

• Dilution :

  Western Blot: 1/500 - 1/2000. IP 1:200-500 ELISA: 1/5000. Not yet tested in other applications.

• Molecular Weight :

  25kD

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  25kD

• Fragment :

  IgG

• Subcellular Location :

  Nucleus . Chromosome, centromere, kinetochore. Cytoplasm . Cytoplasm, cytoskeleton, spindle pole. Recruited by MAD1L1 to unattached kinetochores (Probable) . Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD. Sequestered to the cytoplasm upon interaction with isoform 3 of MAD1L1 (PubMed:19010891) . .

• Other Product Names :

  MAD2L1; MAD2; Mitotic spindle assembly checkpoint protein MAD2A; HsMAD2; Mitotic arrest deficient 2-like protein 1; MAD2-like protein 1

• Gene ID (Human) :

  4085