Topo IIα rabbit pAb

• Background :

  This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also pla

• Description :

  This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also pla

• Product Name Alternative :

  TOP2A; TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II; alpha isozyme

• UniProt :

  P11388

• Swiss Prot :

  P11388

• Reactivity :

  Human; Monkey

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human TOP2A. AA range:1-50

• Target :

  Topo IIα

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; IHC; IF; ELISA

• Concentration :

  1 mg/ml

• Dilution :

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

• Buffer :

  -20°C/1 year

• Molecular Weight :

  174kD

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  174kD

• Fragment :

  IgG

• Subcellular Location :

  Cytoplasm . Nucleus, nucleoplasm . Nucleus . Nucleus, nucleolus .

• Other Product Names :

  TOP2A; TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II; alpha isozyme

• Gene ID (Human) :

  7153