DNA pol ι rabbit pAb

• Background :

  Catalytic activity:Deoxynucleoside triphosphate + DNA (n) = diphosphate + DNA (n+1) ., cofactor:Magnesium., domain:The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity., function:Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity., similarity:Belongs to the DNA polymerase type-Y family., similarity:Contains 1 umuC domain., subcellular location:Accumulates at replication forks after DNA damage., subunit:Binds REV1L (By similarity) . Binds POLH., tissue specificity:Ubiquitous. Highly expressed in testis.

• Description :

  Catalytic activity: Deoxynucleoside triphosphate + DNA (n) = diphosphate + DNA (n+1). Cofactor: Magnesium. Domain: The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity. function: Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity. similarity: Belongs to the DNA polymerase type-Y family. similarity: Contains 1 umuC domain. subcellular location: Accumulates at replication forks after DNA damage. subunit: Binds REV1L (By similarity). Binds POLH. tissue specificity: Ubiquitous. Highly expressed in testis.

• Product Name Alternative :

  POLI; RAD30B; DNA polymerase iota; Eta2; RAD30 homolog B

• UniProt :

  Q9UNA4

• Swiss Prot :

  Q9UNA4

• Reactivity :

  Human; Rat; Mouse

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human POLI. AA range:641-690

• Target :

  DNA pol ι

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; ELISA

• Concentration :

  1 mg/ml

• Dilution :

  Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

• Buffer :

  -20°C/1 year

• Molecular Weight :

  85kD

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  85kD

• Fragment :

  IgG

• Subcellular Location :

  Nucleus . Binding to ubiquitin mediates localization to replication forks after UV-induced DNA damage. .

• Other Product Names :

  POLI; RAD30B; DNA polymerase iota; Eta2; RAD30 homolog B

• Gene ID (Human) :

  11201