EasyStep Human HBP (Heparin-Binding Protein) ELISA Kit

• Description :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HBP, and the Human HBP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human HBP. After TMB substrate solution is added, only those wells that contain Human HBP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBP in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  AZU; AZAMP; AZU1; HBP; HUMAZUR; NAZC; CAP37; Cationic Antimicrobial Protein 37; Heparin-Binding Protein; Neutrophil Azurocidin

• Reactivity :

  Human

• Field of Research :

  Enzyme & Kinase; Infection immunity

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HBP, and the Human HBP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Human HBP. After TMB substrate solution is added, only those wells that contain Human HBP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBP in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  1.5h

• Standard :

  2500 pg/mL

• Sample Type :

  Serum

• Detection Range :

  39-2500 pg/mL

• Sensitivity :

  39 pg/mL