EasyStep Human LpPLA2 (Phospholipase A2, Lipoprotein Associated) ELISA Kit

• Description :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LpPLA2, and the Human LpPLA2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human LpPLA2. After TMB substrate solution is added, only those wells that contain Human LpPLA2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LpPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  PLA2G7; PAF-AH; PAFAH; Lp-PLA2; LDL-PLA2; Platelet Activating Factor Acetylhydrolase, Plasma; Phospholipase A2, Group VII; LDL-associated phospholipase A2

• Reactivity :

  Human

• Field of Research :

  Enzyme & Kinase; Metabolic pathway; Infection immunity

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LpPLA2, and the Human LpPLA2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Human LpPLA2. After TMB substrate solution is added, only those wells that contain Human LpPLA2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LpPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  1.5h

• Standard :

  50 ng/mL

• Sample Type :

  Serum, plasma

• Detection Range :

  0.78-50 ng/mL

• Sensitivity :

  0.78 ng/mL