EasyStep Human AngII (Angiotensin II) ELISA Kit

• Product Name Alternative :

  Ang-II; Angiotensin-2

• Reactivity :

  Human

• Field of Research :

  Endocrinology; Cardiovascular biology

• Assay Type :

  Competitive Inhibition

• Assay Principle :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AngII antigen, and the Human AngII standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Human AngII to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human AngII in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  1.5h

• Standard :

  1000 pg/mL

• Sample Type :

  Serum, plasma

• Detection Range :

  15.63-1000 pg/mL

• Sensitivity :

  10.63 pg/mL