EasyStep Human MMP9 (Matrix Metalloproteinase 9) ELISA Kit

• Description :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMP9, and the Human MMP9 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MMP9. After TMB substrate solution is added, only those wells that contain Human MMP9 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMP9 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase

• UniProt :

  P14780

• Reactivity :

  Human

• Field of Research :

  Enzyme & Kinase; Tumor immunity; Infection immunity; Cardiovascular biology; Hepatology

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMP9, and the Human MMP9 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Human MMP9. After TMB substrate solution is added, only those wells that contain Human MMP9 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMP9 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  1.5h

• Standard :

  80 ng/mL

• Sample Type :

  Serum, plasma

• Detection Range :

  1.25-80 ng/mL

• Sensitivity :

  1.25 ng/mL