EasyStep Human GDF15 (Growth Differentiation Factor 15) ELISA Kit

• Description :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GDF15, and the Human GDF15 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human GDF15. After TMB substrate solution is added, only those wells that contain Human GDF15 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GDF15 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  PDF; MIC1; NAG-1; PLAB; PTGFB; Macrophage inhibitory cytokine 1; NSAID-activated gene 1 protein; Placental bone morphogenetic protein; Prostate differentiation factor

• UniProt :

  Q99988

• Reactivity :

  Human

• Field of Research :

  Cytokine; Apoptosis; Tumor immunity; Infection immunity

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GDF15, and the Human GDF15 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Human GDF15. After TMB substrate solution is added, only those wells that contain Human GDF15 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GDF15 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  1.5h

• Standard :

  3000 pg/mL

• Sample Type :

  Serum, plasma

• Detection Range :

  46.88-3000 pg/mL

• Sensitivity :

  5.22 pg/mL