EasyStep Human EGFR (Epidermal Growth Factor Receptor) ELISA Kit

• Description :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGFR, and the Human EGFR standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human EGFR. After TMB substrate solution is added, only those wells that contain Human EGFR and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EGFR in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  ErbB-1; ErbB1; ER1; ERB-B1; MENA; HER1; Erythroblastic Leukemia Viral (v-Erb-B) oncogene Homolog, Avian; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

• Reactivity :

  Human

• Field of Research :

  CD & Adhesion molecule; Tumor immunity

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGFR, and the Human EGFR standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Human EGFR. After TMB substrate solution is added, only those wells that contain Human EGFR and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EGFR in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  1.5h

• Standard :

  10 ng/mL

• Sample Type :

  Serum, plasma

• Detection Range :

  0.16-10 ng/mL

• Sensitivity :

  25 pg/mL